Abstract:56 Jilin soybean cultivars released in recent years were analyzed by SSR molecular marker technology.The purpose is to understand the trend of genetic diversity of released soybean cultivars from Jilin province in recent years,to offer reference for studying,using and enhancing of Jilin soybean cultivars.The number of total alleles and uniqe alleles of cultivars from Jilin(76,12) was smaller than that from other provinces(78,18) among 15 SSR loci.The genetic diversity indices of Jilin released soybean cultivars(0.63,1.23) were all lower than other provinces(0.76,1.55),the differences were greatly significant(0.0003,0.0002).The results of cluster analysis and principal component analysis indicated that,the genetic distances of the cultivars from Si-chuan,Shanxi and Neimenggu were farther than that from the other provinces.By comparing the average genetic coefficient of different years,we knew the trend of genetic basis of released soybean cultivars,which indicated that the genetic basis of released soybean cultivars from Jilin tends narrow.It is necessary to broaden its genetic basis by introducing diversed and distant cultivars as parents in breeding program from other provinces.