The genetic integrity of 30 accessions of maize(Zea mays L.) stored in national genebank of China was investigated by 70 SSR primers.Each accession was represented by two sub-populations regenerated in different year.The genetic similarity between the two sub-populations of the same accession is all above 0.80,except for 0.77 in Hongbaogu(accession number 00230080).Cluster analysis based on the similarity coefficient showed that Er-Huang(accession number 00210055) and HongBaogu diverged from each other,falling into two branches,and that each one cluster was created by two sub-populations of the same accessions,which were multiplied in different years.The reasons for the cluster divergence and feasibility of SSR for genetic integrity analysis in maize were discussed.