In order to study the genetic diversity of wheat landraces in southwestern winter wheat region and provide basic information for improving wheat quality,560 wheat landraces in southwestern winter wheat region(Yungui plain vice area,Sichuan valley vice area) were analyzed using SDS-PAGE for the allelic variance of high molecular weight glutenin subunits(HMW-GS).The results showed that there were 22 alleles on Glu-1 loci,including 4 alleles on Glu-A1 locus,11 alleles on Glu-B1 locus,7 alleles on Glu-D1 locus.The frequencies of null,7 8 and 2 12 alleles were the highest on the Glu-A1,Glu-B1 and Glu-D1 loci,which were 89.64%,68.21% and 96.43% respectively.There were 46 subunit composition types,and predominant were null/7 8/2 12 and null/7 9/2 12 types with frequency 50.89% and 11.79%.The landraces with elite subunits of 1,2*,17 18,14 15 and 5 10 were selected and 52 landraces contained elite subunit combinations.