SC704 is the major maize variety in Xinjiang over the years.To evaluate the genetic variation of the variety from different regions,total 46 accessions(including SC704 and parents ZPL773,ZPL717) come from several major maize production regions in Xinjiang was identified by SSR markers.The results showed that heterozygosis and variation at some loci were detected in part ZPL717 accessions.Higher genetic uniformity was showed in most ZPL773 and SC704 accessions respectively,but separate heterogeneous accessions were detected.Additionally,94% of SSR loci are same between ZPL773 and B73,and only 41% are same between ZPL717 and Mo17 in this study.Interbreeding and natural mutation accumulation may be major factors in genetic variation among the hybrid and parents.