A total of 253 major wheat cultivars grown in Huanghuai wheat production area at different periods were screened for the starch synthesis genes by molecular markers. The results indicated that 8 of these cultivars were the null Wx-B1 type, and they were used to identify the amylose content. The results showed that significant variation was observed. The range of amylose content was 19.9%-33.3%，only Yumai47,83S502 and Zhongyu 5 with null Wx-B1 was below 27%.The 253 wheat cultivars were also screened for the SSⅡ gene mutations with three STS specific markers published in previous researches．The results indicated that 253 wheat cultivars were all wild type.