One hundred and eighty six soybean germplasms were screened for resistance to adult-plant and seed coat mottling by the inoculation of strain SMV1, and the coincident rate between adult-plant resistance, seed coat mottling resistance to SMV and linkage SSR markers were evaluated. The results showed that among these 186 germplasms, 38 were resistant to both adult-plant and seed coat mottling; 149 were only resistant to adult-plant; 45 were only resistant to seed coat mottling; And 26 were both susceptible to adult-plant and seed coat mottling. 6 SSR markers, Sat_229, Sat_317, Satt335, Satt160, Satt516 and Sat_309, related to SMV adult-plant resistance, were used to identify the 186 soybean germplasms. The coincident rates between the SSR alleles and the resistant presence were 68.9％, 74.3%, 71.1%, 69.8%, 77.4% and 68.2%， respectively; 8 SSR markers, Sat_297, Sat_229, Sat_317, Satt335, Sct_188, Satt160, Satt516 and Sat_133, related to SMV seed coat mottling resistance, were used to identify the 186 soybean germplasms, the coincident rates of Sat_317 alleles with the resistant presence were 79.1％, and that of Sat_229, Satt335, Satt516 and Sat_133 were all over 70％. These markers related to SMV1 resistance were available for the marker assisted selection for SMV resistance breeding.