Germination percentage of 89 accessions wheat germplasm stored-4～0℃ packed in vacuum aluminum foil bag and non-vacuum aluminum box were tested.Disussed the effect of storage method form three levels：the difference of germination percentage of two package methods for seeds stored at low temperature and initial germination percentage before stores, the difference of germination percentage of two package methods for seeds between storage years and the difference of germination percentage between two package methods in the same year. The result showed：the viability of wheat germplasm stored at low temperature were tested, the 82 percent of materials of mean germination percentage was higher than initial germination percentage,but ten years later, germination percentages were obviously decreased than initial germination percentage,and the germination percentage of aluminum foil bag were reduced of less than aluminum box and the difference was significant, the method of sealing up in aluminum foil bag showed best effect.