Two species of tropical fruit in Sapotaceae, sapodilla (Manilkara zapota (Linnaeus) P. van Royen) and egg-fruit-tree (Pouteria campechiana (Kunth) Baehni), were used as materials, the chromosome number and karyotype were studied. Results showed that：Chromosome number of sapodilla is 26, that of egg-fruit-tree is 28. The karyotype type of the two species are “2B” and “1A” respectively, karyotype formula are“18m＋8sm”and“26m＋2sm”. The asymmetry index and the constitution of relative length of the two species are very different.