Seed oil tocopherol content in 50 divergent genotypes of oilseed rape of Brassica campestris L., Brassica napus L., Brassica juncea Coss.and Eruca stiva Mill. were analysed by HPLC method, the results indicated thatα-tocopherol andγ-tocopherol are the two dominant tocopherols in seed oil, and significant variation in both composition and content could be found among different genotypes. tocopherol content in seed oil of Brassica napus L. is the highest among the species, with a average content of 123.11 mg/100g oil, the genotype Omega has the highest total content of 144.73 mg/100g oil, the highest ratio of α-tocopherol andγ-tocopherol is 0.77. α-tocopherol ,γ-tocopherol and total tocopherol content showed significant negative correlation with carotenoid content, a significant positive correlation were found betweenα-tocopherol content and oil content. α-tocopherol ,γ-tocopherol and total tocopherol content presented significant positive correlation with growth peroid, α-tocopherol and total tocopherol content are significantly correlated with plant height, total tocopherol content demonstrated significant positive correlation with 1000 seeds weight. However, α-tocopherol ,γ-tocopherol and total tocopherol content showed no correlation with number of siliques and Seeds number per silique.