45 crisp peach varieties were used as plant material in this paper. A total of 152 alleles were amplified by 25 primer pairs distributed on eight linkage groups on Prunus reference map. 146 alleles showed polymorphism,and the percentage of polymorphic bands (PPB) was 96.05%. The Nei′s gene diversity index (He) and Shannon′s information index（H0）of crisp peach varieties were 0.2283 and 0.3609,respectively. Crisp peach varieties from the area along Yangtzi river had the highest Nei′s gene diversity index (0.2211) and Shannon′s information index(0.3476),followed by subtropical zones in South China and YunnanKweichow Plateau ,the lowest from the North China Plain. Groups cluster analysis based on the UPGMA method indicated that there was some relationship between crisp peach varieties and ecological zones,but not all varieties were in comformity with geographic origin and some from different ecological zones overlapped. Crisp peach varieties from the Yangtzi River and YunnanKweichow Plateau had closer genetic relationship,followed by subtropical zones of South China,as far as the North China Plain. The results preferred the opinion that the crisp peach varieties from Yangtzi River may not only origin from the north China,but also from the YunnanKweichow Plateau and subtropical zones in South China.