Using 80 SSR markers, 192 wild soybean genotypes from Jiangxi province in China, were analyzed for genetic diversity. The results showed that total 343 alleles were identified, with 7.2 of the average of alleles per locus; the average genetic diversity index was 0.7369; the average polymorphism information content (PIC) was 0.7060. All these results indicated the abundance of genetic diversity among these genotypes. At the same time, genetic diversity were analyzed for genotypes from different latitude and altitude, which of circumpolar latitude and low altitude were higher. All the 192 wild soybean genotypes for molecular clustering could be clustered into 3 categories. It was found that the genetic distance was approximately consistent with the origin of the wild soybean genotypes.