The pathogens of Phytophthora parasitica var. nicotianae from sisal was successfully isolated by pure-culture. Then 38 agave accessions were analyzed to evaluate their resistance to Phytophthora parasitica var. nicotianae through artificial inoculation. The result showed that the accessions with high resistance were A. americana, A. hybrid D.h.No.368, Moyin No.6, Moyin No.12, Moyin No.7, Moyin No.5, Jia No.7, A.cantala Roxb., A.Potalorum Var.a.h‘Dongfang hong No.109', A.attenuata var and Moyin No.4. And disease severity and the speed of disease spot development can be used as indexes for rapid resistance identification of agave.