Huperziaceae has attracted the attention of researchers in recent years because of its Huperzine A (HupA) being a potent, reversible, and selective acetylcholinesterase inhibitor (AChEI) . HupA in four plants of Huperziaceae from Wuyi mountain were determined by HPLC. The results showed that the Hup A were found in all four species（both two genus）, with the highest in Phlegmariurus petiolatus(0.151%)and lowest in Huperzia sutchueniana(0.016%).The comparisons of content of Hup A in Huperzia serrata(Thunb. ex Murray) Trev. var. longipetiolata(spring)H.M.Chang among 11 populations from this area were carried out in this paper ,which indicated that Wuping and Sanyuan population had good performance.Hup A in some species near Huperziaceae from this area were also determined .It is suggested that there were excellent resources in this area and there is no HupA in Lycopodiastrun casuarinoides.The fitful plant of Hup A resource for cultivation was also discussed here.