A cold and freezing-induced full-length cDNA library of Ammopiptanthus mongolicus, a plant with remarkable resistance to severe environments, was constructed by using SMART (Switching mechanism at 5’-end of RNA transcript)cDNA library construction method. The titer of the unamplified library is 9.44?06 pfu/mL; the recombination percentage of the library is 98.3%; the sizes of the insert cDNA fragments ligated to λTriplEx2 vector ranges from 0.5to 2.5b and averagely reaches up to 1.0kb. The titer of the amplified library is 1.98?010 pfu.mL-1. Four hundred and eighty positive clones selected from the unamplified library were sequenced and some stress responsive related genes and novel genes were found. Moreover, using the amplified library as template, a RD22-like gene with the complete coding region of 1083 base-pair was obtained by PCR method. All of the results indicated that the library quality is high and can satisfy the demands of follow-up researches.