TILLING(Targeting Induced Local Lesions In Genomes) is a complete reverse genetics approach for rapid and high throughput detecting of the target point mutations induced by chemical mutagen—Ethyl Methane Sulfonic acid(EMS). This paper briefly introduced the application status of TILLING on main crops, i.e., rice, wheat, corn and soybean, etc, with the emphasis on a review of the advances in methodology of mutagenized population construction and novel mutant alleles detect. The problems and future prospects of TILLING were also discussed.