To optimize the concentrations of CuSO4 and iron source used in wheat embryo culture, investigation on the influence of Fe and Cu ions in culture media on wheat regeneration was performed using CB037, Xinchun9, and Bobwhite as materials. The results showed that the the efficiencies of embryogenic callus induction and green shoot induction has not difference when the concentration of CuSO4 was from 0.1 to 4.1 祄ol L-1, and 6.1 祄ol L-1 was toxic for mature embryos of wheat. Regeneration efficiency from the mature embryos of CB037 was obviously improved on the media containing 40 祄ol L-1 iron source. The optimal concentration of iron source were 40 to 50 祄ol L-1 when the immature embryos of CB037 were used as explants, both of callus differentiation and green shoots induction in this level were higher than them in the level of 20 祄ol L-1(at random). For the immature embryo culture of Xinchun9 and Bobwhite, the ideal concentration of iron source was 20 祄ol L-1. The sensibility of different genotypes to iron source is discrepant. In average, the order for regeneration potential of immature embryos among the three wheat genotypes used in this study is CB037, Xinchun 9 and Bobwhite.