MAPKs are of importance in stress signal transduction process in plant. In order to investigate the function of wheat MAPKs, a MAPK gene named TaMAPK2, was isolated from wheat. To obtain the polyclonal antibody of TaMAPK2, the non-conservation fraction of TaMAPK2 gene was constructed into the prokaryotic expression vector pET-28a-(+). Under the condition of 1 mmol/L of IPTG, the expression of the fusion protein His-antiMAPK2 was up to the peak. The fusion protein His-antiMAPK2 was purified by HisTrapTMHP and used to prepare antibody. The titer of the rabbit`s anti-serum was measured by ELISA method. The rabbit` antiserum with high titer (>80000) was obtained. The polyclonal antibodies can be used for further investigation, which establish the foundation for investigating the function of the MAPK2 gene in protein level.