The genetic diversity of 96 landraces of Tangshan and Tianjin auzuki bean was investigated by SSR molecular markers . These materials can be divided into 14 populations in terms of their geographical origins. 89 out of 197 pairs of primers were detected producing 285 bands , with total percentage of polymorphic bands (PPB) was 100%. The genetic diversity of ‘Zunhua’ population was the highest and that of Yutian and Qian’an was second higest. All landraces from Tianjin excluding Jinghai population has relatively low genetic diversity and the lowest PPB with 22.47% can be found in Ninghe polpulation. Total of 96 accessions are divided into three groups by genetic similarities. Jinghai population from Tianjin was included by Tangshan auzuki bean and Dagang population from Tianjin was one separate group. The rest accessions of Tianjin auzuki bean froms another group. So reference for landraces conservation and selection of diverse parental combinations can be provided by this research.