Sequence related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversities of 13 pummelo (C.maxima) germplasms collected from southern Zhejiang province and Guanxi miyou pummelo and its bud mutant from Fujian province. Fourteen SRAP primer combinations were thus selected and a total of 220 PCR fragments with an average of 15.7 bands per primer combination were obtained. Among these bands, 122 (55.4%) were polymorphic，showing a relatively low level of genetic diversity among the analyzed germplasms. Eleven genotypes were uniquely identified in the 15 germplasms by SRAP markers. UPGMA Clustering analysis of the SRAP banding patterns showed that the 15 pummelos could be divided into 8 major groups at the similarity coefficient level of 0.97. The first group included the 7 Sijipao varieties. The second group included Guanxi miyou and its bud mutant hongrou miyou. Each of the rest pummelos, i.e., Pingyang pummelo, Zaoxiang pummelo, Chuhong pummelo, Hongxin 1 pummelo, Hongxin2 pummelo and Sour pummelo, was clustered as a separate group. It showed that slight change occurred at DNA level in Wucheng1, Wucheng3, Mazhanhongxin pummelo based on genetic diversities.