Abstract: The characteristic of starch RVA, dough mixing and protein of 698 germplasm including 412 landraces, 154 improved varieties and 132 introduced varieties were studied in detail in two years, and the quality characteristic of different wheat ecological types was also analyzed. The results are as follows: 1) Different source germplasm showed different quality characteristic. Long dough mixing development time and high Zeleny sedimentation were found in improved variety and introduced variety. There were 10 and 16 germplasm with long mixing development time over 3.5min, 32 and 25 germplasm with high sedimentation over 40.0 ml of this two type variety respectively, and the maximum development time was 5.2min , maximum Zeleny sedimentation was 61.8 and 68.8 ml respectively. Good starch RVA characteristic and high protein content were showed in landrace. There were 24 landraces with high peak viscosity over 2900cP, and the maximum was 4099cP.The 48.8% landrace showed the sedimentation over 40.0ml, and the maximum was high to 58.8ml. 2) Different characteristic was found in landraces belonging to different ecological types. Good starch RVA characteristic and high protein content were showed in landrace in Yantai and Qingdao belonging to Jiaodong hilly land, the variety belonging to winterness late-maturing type. Good starch RVA characteristic was found in landrace in Binzhou,Weifang and Dezhou belonging to plain in northwestern Shandong province, and the variety belonging to winterness/semiwinterness late-maturation type. High protein content was found in landrace in Linyin and Taian belonging to hillyland and mountain in mid-Shandong province, and the variety belonging to semiwinterness/winterness type. 3) Some germplasm with outstanding quality characteristic were selected, including improved varieties Jinan17, Jinning16, Jimai20, Zhouyuan9369 and Taimai1 with Zeleny sedimentation over 50.0ml and development time over 4.5min, induced varieties Gaocheng9415, Ji5099, Linfen6410 and Linfen6510 with Zeleny sedimentation over 60.0ml, landraces Banjiemang, Baitutou, Sanbamai, Xiaobaimang and Banjiesui with high peak viscosity over 3100cP, and landrace Mazhatou, Baidu, Baiqisiwu, Yisuishou, Silengbai, Baisuihong and Banjiehongsui with high Zeleny sedimentation over 50.0ml. All these germplasm could be used as the preferred option parent in screening quality wheat.