With manual pollination, an interspecific hybrid was obtained from the cross between a pungent frutescens accession H101 (P1) and the pungent C. chinense accession PI439487 (P2). 25 phenotypic characters were observed and compared among P1, F1 and P2. The results indicated the growth vigor of F1 had heterosis significantly, and the majority of the other phenotypic characters were intermediate to that of either parent. SRAP analysis showed that the Fl hybrid contained 220 bands of two parents , accounting for 53.92% of the total loci, and the percentage of 143 bands holding by F1 and either parent reached to 35.05%. The number and the percentage of the specific loci in F1 were 12 and 2.95％ respective1y. The genetic similarity coefficient between F1 and P1 was 0.749, greater than that between F1 and P2 (0.740), suggesting the hybrid was more similar to the female parent at DNA level.