majing
College of Horticulture and Landscape, Southwest University, Chongqing Engineering Research Center for Floriculture,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of EducationThe National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)
The promoter fragments of 1298bp and 838bp upstream from the 5’ upper of Chimonanthus praecox non-specific lipid transfer protein(nsLTP) gene family member CpLTP3 and CpLTP4 were isolated from the genomic DNA of Chimonanthus praecox by hiTAIL-PCR technique respectively. Bioinformatics analysis revealed that the two sequences contained basic cis-elements,such as TATA-box and CAAT-box and many elements involved in the plant abiotic stress. The result of transient expression indicated that all the sequences had the function to drive reporter gene GUS in tobacco.