The promoter fragments of 1298bp and 838bp upstream from the 5’ upper of Chimonanthus praecox non-specific lipid transfer protein(nsLTP) gene family member CpLTP3 and CpLTP4 were isolated from the genomic DNA of Chimonanthus praecox by hiTAIL-PCR technique respectively. Bioinformatics analysis revealed that the two sequences contained basic cis-elements，such as TATA-box and CAAT-box and many elements involved in the plant abiotic stress. The result of transient expression indicated that all the sequences had the function to drive reporter gene GUS in tobacco.