The chromosome number and karyotype of three Zantedeschia Cultivars were studied using squash method. The results showed that the chromosome number of all tested cultivars was 32. Karyotypes among them were not distinct with mostly metacentric or submetacentric chromosomes. The karyotype formula of three Zantedeschia hybrid cultivars as follows：‘Allure’ was 2n= 2x= 32= 14m（2SAT） 2sm；‘Cupdio’ was 2n= 2x= 32= 14m 2sm； ‘Odessa’ was 2n= 2x= 32= 1M 15m（1SAT）. All cultivars belonged to 1A type and its asymmetry index were 72.96%, 77.39%, 75.93% respectively.