SSR methods were applied to detect genetic diversity of 45 Tsai-tai germplasm from China, provided biological foundation for the utilization and seed breeding of the Tsai-tai germplasm. 124 bands were amplified with 63 SSR primers.The amplified DNA fragments size were 100~300bp. Similarity coefficients between 0.56 ~ 0.89. The results show there were abundant genetic diversity in the Tsai-tai germplasm of China.