The systematic survey was made to the agro-biological resources and related traditional knowledge of the Nu minority in 4 townships in Lanping,Gongshan and Fugong county, Nujiang Lisu autonomous prefecture,yunnan province.The results show that there were 121 ago-biological resources involved in the production and daily life of the Nu minority in the survey area,which belong to 24 families,49 species, including 58.68% food crops, 19.83% vegetables, 15.70% fruit trees, and 5.79% medicinal plants. The traditional knowledge of the agro-biological resources utilization by Nu Nationality were mainly related to food, herbal medicine, festival, religious worship and culture. Most of the resources are used for food and herbal medicine. The special usage and processing methods possess Local and Nu’s characteristics. he agro-biological resources not only provide Nu minority for basic survival food and medicine,but also play an important role their religious activities.