In order to investigate the difference of seed storage proteins in Faba bean, Seed storage proteins extracted from ten species of Faba bean were analyzed with SDS-PAGE, the result showed that it is different among the seed storage proteins of faba bean, a total of 23 subunits of different Rf were detected, counting 13 polymorphic protein subunits. The similarity index of seed storage proteins subunits in Faba bean is 0.619~0.947, with an average of 0.744. Cluster analysis divided the accessions into three major clusters in the leave of 0.8260 GS, the second cluster showed higher polymorphism, and 7 pair Faba bean were identified as the parents.