Aggregation breeding method was used to create the new pumpkin germplasm with high inosital that was analyzed with the HPLC technique. A new pumpkin cultivar ‘Yichuanhong’ was obtained. The contents of inositol and polysaccharide in the fruits of ‘Yichuanhong’ were 661mg/100g and 45.8mg/g, which were 120% and 30% higher than CK cultivars, respectively. The results also showed that this new cultivar had low reductive sugar, less than 50 mg/g. Therefore, it is suitable to be used in the daily diet treatment by the people with diabetic, hypertention, or heart disease. Besides, ‘Yichuanhong’ has good eating quality, high yield, and diseases resistance. This cultivar can be cultivated in both spring and autumn. ‘Yichuanhong’ has greatly increased the economic value of pumpkin as a traditional vegetable, and provided a safe and effective means to prevent diabetic, hypertention, or heart diseases.