This study was conducted to indentify the resistance against root-knot nematode in 120 kenaf varieties using field trials and pot culture. The results showed that the main species of root-knot nematode in the field were Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenria. 5, 19, 25 and 71 varieties of 120 tested varieties were of high susceptibility, middle susceptibility, middle resistance and high resistance to these root-knot nematodes, respectively. Further study was carried out in representative 22 varieties using pot culture, 7 varities were middle resistance to Meloidogyne incognita, but no highly resistant or immune varieties.