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Home > Archive>Volume 14, Issue 1, 2013 >181-185. DOI:10.13430/j.cnki.jpgr.2013.01.027 Online First
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Development and Characterization of PvPGIP2 Transgenic wheat Plants with enhanced resistance to Rhizoctonia cerealis
DOI:
10.13430/j.cnki.jpgr.2013.01.027
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  • Zhang Zeng yan

    Zhang Zeng yan


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    Abstract:

    Polygalacturcuase-inhibing proteins (PGIPs) are defense proteins produced in plants, which can inhibit the growth of pathogenic fungi. In this study, pA25-PvPGIP2, the transformation vector of a bean PGIP gene PvPGIP2, which can be highly expressed in monocot plants, was correctly constructed. In the pA25-PvPGIP2 vector, PvPGIP2 gene was driven by maize ubiquitin promoter. 4000 of embryo callus of Yangmai 18 were bombarded by the particle containing pA25- PvPGIP2. Among the regenerated 203 plants, 65 positive transgenic individuals were identified through PCR assay in the T0 generation with a transformation frequency of 1.625 %. The transgenic wheat plants from T0 to T2 generations were subjected to PCR, RT-PCR, and Q-RT-PCR, and inoculations with Rhizoctonia cerealis as well as the disease resistance tests. Results showed that the alien PvPGIP2 gene was introduced into these transgenic wheat plants, can be inherited from T0 to T2, and expressed in the wheat background. The transgenic wheat plants expressing PvPGIP2 showed enhanced resistance to R. cerealis compared with untransformed Yangmai 18.

    Key words:PvPGIP2 gene ; Transgenic wheat; Rhizoctonia cerealis; Resistance
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History
  • Received:March 26,2012
  • Revised:November 15,2012
  • Adopted:December 19,2012
  • Online: January 15,2013
  • Published:
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