The wild Fragaria species and their geographic distribution were investigated in Sichuan province and neighboring regions (excluding Qinghai province). The wild strawberry resources were collected and moved into the conservations. The variance and principal component analysis of the botanic characters, flowering and fruiting habits, resistant capability under abiological and biological stress were evaluated respectively. The results indicated that the wild strawberry resources in Sichuan and neighbouring areas distributed mainly from 26014′～33022′N to 96057´～108013´E at the altitudes of 823～4531m. Four wild strawberry species including Fragaria. nilgerrensis Schlecht., F. pentaphylla Lozinsk. (red fruit type and white fruit type), F. moupinensis (Franch.) Card. and F. gracilis Lozinsk. hadve been identified in the investigatedion regions. The fruit of F. nilgerrensis Schlecht. has special peach aroma. The plant height, petiole length, leaf length and width, hairs density, drought resistance and high temperature resistance wrer the key indicator of the hereditary character. And hairs density was closely related to the stress resistance.