In this study, 134 pairs of primers were designed by screening the InDel loci at a distance of every 1M-3M base pairs on all chromosomes of cucumber based on genome resequencing. The effectiveness of the InDel markers were tested on 16 typical cucumber germplasm. The results showed that distinct PCR amplification products were obtained from the InDel markers. Among these markers, 116 InDel were polymorphic, accounting for 86.6% of the total and revealing the genetic diversity and specificity of the tested cucumber germplasm. The InDel markers developed in the study will offer powerful tools for cucumber germplasm identification and marker-assisted selectionin cucumber breeding.