Brassica oleracea cytodeme includes variant wild types, which are important resources for improving cultivated types of B. oleracea. During the long evolution, reproductive isolations were formed among variant type of B. oleracea cytodeme at certain degree. In the present study, 35 lines from seven wild and eight cultivated types were crossed randomly to each other in order to investigate the crossability in B. oleracea cytodeme. Significant differences for crossability were detected among crosses, and biological type, cross direction, and genetic difference were found to affect the crossability significantly. Our data suggests that the crossability of combinations with wild type as female is higher than those with wild type as male in the crosses between wild and cultivated types of B. oleracea.