The phloem proteins play important role in the maintenance of morphology, material transportation and wound protection in higher plant. Here, cDNA sequences of phloem protein were obtained using RT-PCR technology from two celery varieties ‘Liu He Huang Xin Qin’ and ‘Mei Guo Xi Qin’. The full-length of the two phloem-protein genes were 546 bp, and encoded 181 amino acid residues, respectively. The nucleotide sequences alignment showed that there were 3 loci differences: 88G/A, 399T/C and 489T/C. The deduced amino acid sequences showed that there was 1 residue difference, 30T/A. Prediction of the proteins molecular weight were 19 kDa, and pI were 9.18, respectively. The phloem-proteins from ‘Liu He Huang Xin Qin’ and ‘Mei Guo Xi Qin’ have highly identity with Lycoris aurea. There were 5 leucine and 4 tryptophan amino acid residues in the conservative position, respectively. Real time RT-PCR analysis showed that the genes expression were tissue specificity, which mainly expressed in the stem and root.