Simple sequence repeats (SSR) markers were adopted to evaluate genetic diversity of 43 accessions of Zhejiang barley landraces with different resistance to Fusarium head blight (FHB). Totally 87 alleles were revealed among the landraces with 29 SSR markers, 2~9 alleles could be detected at different SSR locus, with average of 3 alleles per SSR marker. The average polymorphism information content (PIC )was 0.3509, Shannon’s information index (I) was 0.6951, Nei’s gene diversity (H ) was 0.4268. Based on the data of SSR, the genetic similarity among 43 barley landraces was calculated. The mean of genetic similarity (GS) was 0.467, varying from 0.082 to 0.986. Cluster analysis showed that all the landraces were divided into 4 groups. Abundant genetic variation was observed among them and the genetic basis of two-rowed accessions varied greater than that of six-rowed ones. Great genetic variation was also deteted in FHB resistant and moderately-resistant landraces. The results of principal coordinates analysis and cluster anlysis revealed that the genetic relationship among these landraces was not in accord with their geographical origins.