Resistance to stalk rot caused by Pythium inflatum Matthews is an important trait for maize breeding. To identify effective resistant germplasm, totally 287 inbred lines representing most of the genetic diversity of Chinese maize inbred germplasm were evaluated for stalk rot resistance during 2010-2011. The results showed that 171 maize inbreds were resistant to stalk rot, among which 43 inbreds were highly resistant to stalk rot, accounting for 14.98%. And 116 maize inbreds were stalk rot sensitive, among which 95 inbreds were highly sensitive to stalk rot, accounting for 33.10%. Lancaster, Reid and P genetic groups were identified to be important sources for maize stalk rot resistance. The results provided useful information for the introduction of maize germplasm and genetic improvement for resistance to stalk rot in the future.