According to the conservative sequences of the NBS-LRR disease-resistant genes (RGAs) , we isolated 10 homologous sequences (pp-01~10) from the mango variety ‘Jin Huang’ genomic DNA. Analysis of DNA sequences showed that the RGAs showed more variation in the 200~300-bp region, and Pi value was above 0.4. The clones of pp-01 to 10 showed great homologous differences, with the similarity from 11.0%~98.4% and divergence value from 1.6 to 1000.7 and 10 RGAs can be divided into two groups. pp-01~10 have an open reading frame, and the encoded protein contains the domain of the P-loop and Kinase-2a that were typical character of NBS gene families. pp-01~10 can be divided into two families, TIR-NBS-LRR and CC-NBS-LRR, by analysis of phylogenetic tree, and the homology was 22~60% compared with NBS genes of other known species.