Isolation and expression analysis of CoPht1; 1 from oil tea
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The National Key Technologies R&D Program of China

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    Abstract:

    The phosphate transporter named CoPht1;1 was isolated from oil tea(Camellia oleifera’Xianglin4’)with the method of RT-PCR and RACE. Its expression under different phosphorus level at different time was checked with qRT-PCR. The CDS of CoPht1; 1 is 1 626 bp in length and it codes a predicted protein of 542 amino acids. The amino acid identity compared with other phosphate transporters is highly conserved and shows the highest similarity(88%)with the CoPht1;1 of Catharanthus roseus. Putative secondary structure and topology of the encoding protein have the main feature of transmembrane protein, and is consistent with Pht1 encoding protein in other species. The real-time RT-PCR result showed that expression of CoPht1;1 is induced by P-deficiency,and varied at different time. The results showed that CoPht1; 1 play an important role in phosphate uptake in camellia.

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History
  • Received:August 03,2012
  • Revised:December 24,2012
  • Adopted:March 11,2013
  • Online: April 02,2013
  • Published:
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