The eggplant male sterile materials developed from advanced backcrossing of interspecific hybrid were used to study the anther’s morpholgy, the stage and the mechanism of pollen abortion by morphological and cytological methods. The results showed that the three eggplant male-sterile materials were grouped into three types: degenerative-anther type, petaloid–anther type and intermediate-anther type, according to the sterile anther’s morphological characters, ProphaseⅠwas the key stage that pollen abortion occurred. The abnormity of tapetum structure and function was probably the common reason that resulted in the male sterility. Abnormal meiosis was the intermediate process of pollen abortion. the transport disruption of polysaccharides may be one of the reasons for its occurrence of male serility.