Different agronomic trait cluster analysis of 11 sweet potato varieties (lines) was done by UPGMA method. Three groups ,which include 2,3,6 varieties respectively, were divided on similarity coefficient of 0.17. SRAP clustering analysis showed that Part II group was divided into two sub-clusters: II A and II B on similarity coefficient of 0.77. II A included three varieties and II B included three varieties also. Part III group was divided into two sub-clusters: III A and III B on similarity coefficient of 0.75. III A included two varieties and III B included two varieties also. Fengshoubai wild-type and it’s mutant M-0 were clustered together in the cluster analysis of agronomic traits and SRAP markers. Six biological traits included leaf shape, vein color, young leaf color, root skin color, root shape and root flesh color were observed and compared between wild type and mutant. The results showed that fengshoubai mutant M-0 did not show significant difference among five traits, but significant difference only on root flesh color. Fengshoubai M-0 is pale orange flesh color while its wild type is white. The percentage of polymorphic bands between fengshoubai mutant M-0 and it’s wild type is 29.49 %, effective alleles are 1.2085,which show there are no obvious genetic differences between them. We preliminary deduced that the mutation was the result from point mutation.