Yu98-8A1, a herbicide-resistant cotton genic male-sterile line, was developed by successive backcrossing cotton line EHC09-002 which has the herbicide-resistant gene EPSPS-G6 to a genic male-sterile line Yu98-8A. Phenotype observation of corrala showed that, compared with homogeneity normal fertile plants, smaller corolla and exposed stigmas were the significant characteristics. Cytological microscopical observation showed that the anther abortion of Yu98-8A1 occurred during the stage of microspore development after tetrad formation, which included that there was no spinescent protuberance on the pollen wall, and the microspore appeared abnormal and break down gradually. PCR identification and glyphosate resistance test indicated that herbicide-resistant gene EPSPS-G6 had been aggregated successfully into Yu98-8A1, and the anti-herbicide concentration reached up to 0.3%. Herbicide resistance makes Yu98-8A1 have significant value in the utilization of cotton heterosis.