Based on 281 individual plants of F2 population derived from a cross “Yuan Naked Oat” x “555”, a genetic linkage map for naked oat (Avena nuda L.) was constructed by 20 pair AFLP primers, 3 SSR primers and 1 panicle type character. The map was 1544.8cM in total length with 20.1cM for the average distance between neighboring markers. 92 AFLP markers, 3 SSR markers and 1 morphological trait were mapped on 19 linkage groups, which contained 2-14 markers and varied in size from 23.7 to 276.3cM with an average of 81.3cM. The segregation ratio of panicle type fitted to 3:1, and 11 AFLP markers demonstrated distorted segregation with the percentage of 11.5%. The results provided a framework of genetic linkage map for naked oat (Avena nuda L.), which is the theoretical basis for QTL mapping, molecular breeding and comparative genome in naked oat research.