Interspecific hybridization is an important approach to broaden genetic base and transfer superior traits, Which has been widely applied for improving crops. The objectives of this study were to obtain novel Brassica rapa by interspecific hybridization between B.juncea and B.rapa and transfer advanced characters from B.juncea, improving agronomic traits and resistance of B.rapa and broaden its genetic base. The results showed that hybridization with B.juncea as the female parent and B.rapa as the male parent was easy to harvest hybrid seeds. F1 plants were sterile and performed strong vegetative growth, which was backcrossed to the B.rapa to produce BC1 progeny. BC1 plants having great diversity of the morphology showed self-incompatible, with the average pollen stainability, being 34.8%, which ranged from 0 to 84%.. Self-compatible plants and pure yellow seeded plants were founded in BC1F1 and BC2 progeny. Moreover, BC1F1 and BC2 population, both showing increased fertility level, had the average pollen stainability of 79.7% and 79.1%, respectively.