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Home > Archive>Volume 14, Issue 5, 2013 >959-964. DOI:10.13430/j.cnki.jpgr.2013.05.029 Online First
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Screening Of SSR Core Primer Pairs for Identification Tobacco
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10.13430/j.cnki.jpgr.2013.05.029
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  • 尹国英

    尹国英


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  • 杨小燕

    杨小燕


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  • 何其波

    何其波


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  • 曲存民

    曲存民


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  • 张建奎

    张建奎


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  • Dai Xiu-mei

    Dai Xiu-mei

    Southwest Univisity
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    Abstract:

    Core primers are important for genetic diversity analysis, germplasm identification, purity and genuineness test, and fingerprint establishment. Currently, we have screened 20 tobacco germplasm stocks with distant relationships by using of 278 SSR primer pairs, which evenly distribute on 24 chromosomes, through the strategy of distant relationships, and among which 32 primer pairs were selected out. And then, another 10 germplasm stocks with similar relationship were used to screen the 32 primer pairs, and 14 SSR core primer pairs were obtained. These 14 SSR core primer pairs were conducted on pedigree research, genetic diversity analysis and agronomic traits clustering study for 39 germplasm stocks. The result showed these core primer pairs could be applied to germplasm identification and genetic diversity study in tobacco.

    Key words:tobacco, SSR, core primers, polymorphism
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History
  • Received:November 14,2012
  • Revised:April 07,2013
  • Adopted:July 09,2013
  • Online: August 08,2013
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