AFLP markers were used to evaluate genetic diversity among 60 tomato inbred lines with good quality. Total 905 bands were amplified using 17 AFLP primer combinations, and 251 bands of them were polymorphic bands. Average number of polymorphic loci detected by per primer combination was 15, and mean polymorphism rate was 27.7%. Unique fingerprints of 60 tomato inbred lines were obtained by a minimum of 2 AFLP primer combinations, E7M4 and E7M10. Genetic similarity coefficients of 60 inbred lines ranged from 0.259-0.952 with a mean of 0.664. 60 tomato materials can be divided into seven groups at similarity coefficients from 0.54-0.57 when UPGMA clustering was used. It was showed clustering results had no pronounced relation with phenotypic traits, such as sources, fruit size, fruit shape, and so on.