The transcription factor corresponding to the mybA that regulates anthocyanin biosynthesis were cloned and analysised on a sample of 14 Chinese wild grapes species. Two complete mybA-related gene sequences, VvmybA1 and VlmybA2 were obtained, and a total of 121 SNPs were detected in the sequenced fragments which giving a high level of polymorphism. Three neutrality tests had been used to compare the patterns of sequence variations, the result indicates a non departure from neutrality expectations for gene VvmybA1 and VlmybA2 of Chinese wild grape which reflected equilibrium between genetic drift and selectively neutral mutation. The mybA gene acquired form different wild accessions represented a highly homology. Yet extensive base deletion, insertion and substitution existed in promotor region, intron region and the third coding exon, which exhibited rich genetic diversity between species of Chinese wild grapes. Furthermore，there were several unique bases or mutations in the mybA gene of wild grapes that might be selected to be the molecular marker to distinguish themselves from others well. Comparing and analysising the gene structure and phylogenetic tree of the gene sequence, the studied Chinese wild grape species could be divided into five groups. Moreover, V.betulifolia and V.hancockii were initially predicted to be primitive in the evolution.