The plant β-1,3-glucanase belongs to one of the important pathogenesis-related proteins (PR-proteins), which could be accumulated when induced by elicitors. The seedlings of peanut cultivar Huayu20 were sprayed with 1.5 mmol/L Salicylic Acid (SA), then the total RNAs were extracted and the mRNA expression amount of β-1,3-glucanase gene were inspected by Real-time PCR. The expression amount of β-1,3-glucanase gene reached the highest after 24h induced by SA, which was 1.8 times higher than that of control not induced by SA. Three specific 5’ upstream primers were designed and synthesized according to peanut β-1,3-glucanase gene cDNA sequences (GenBank JQ801335), and the PCR amplification were conducted using the genomic DNA of Huayu20 as the template by TAIL-PCR method. A 973bp upstream promoter fragment was obtained and submitted in NCBI (GenBank KC290400), named by Ah-Glu-Pro. Promoter sequence analysis by PLACE and PlantCARE showed that the 973bp sequence contained some typical cis-elements, such as TATA box, CAAT box, pathogen and SA cis-acting regulatory element. A 931bp fragment was obtained and named Ah-Glu-P, using a pair of primers designed according to Ah-Glu-Pro. Ah-Glu-P then was inserted into pCAMBIA1301, replacing its CaMV35S promoter. The recombinant plasmid was named pCAMBIA1301-Ah-Glu-P and then transferred into onion epidermal cells by Agrobacterium EHA105-mediated transformation. GUS staining on the onion epidermal cells was conducted after 48h induced by 5.0 mmol/L SA. The transformed onion epidermal cells appeared blue when induced by SA, while the control cells not induced by SA didn’t appear blue, which indicated that the Ah-Glu-P could be an inducible promoter and might contain responsive element relative to SA.