In order to explore new wheat germplasm for wheat quality improvement, high molecular weight glutenin subunits (HMW-GS) composition and quality properties were analyzed among 67 American wheat accessions. SDS-PAGE were used to detect HMW-GS compositions and the results showed that there were 20 types of subunits and 25 types of subunit combinations, meaning that Glu-1 locus had a high level of variations. The frequencies of quality subunits such as 1, 2* and 5+10 were 16.4%, 35.8%, 74.6% respectively, higher than any other subunit. The quality score ranged from 5 to 10, with an average of 8.2. Forty one accessions scored above 8(including 8), and even 8 accessions had a score of 10. The quality properties analysis was treated as preliminary analysis, and the results indicated that these materials had lower quality indexes. Base on the results above, these American accessions could be parental materials to improve the HMW-GS compositions of Huang-huai wheat regions of China .