Eight morphological characteristics on leaf and inflorescence of 31 chrysanthemum species were recorded, and converted to the corresponding code according the method of characteristics evaluation and classification in guidelines for the conduct of tests for distinctness, uniformity and stability of chrysanthemum. Depend on these code of characteristics and 7 quantitative characters calculated from 14 morphological traits, the phylogenetic relationship of 31 species form Chrysanthemum and Ajania were analyzed respectively through clustering analysis using SAS software, So as to assess the effectiveness of the method compared with traditional morphological classification and other results of clustering analysis depend on molecular markers, the result showed that, relevant multiple both qualitative traits from DUS test and quantitative traits from leaf covers large amount of diversity, could be compare the similarity of species from Chrysanthemum and Ajania, is able to accurately reflect the genetic relationship between species. and results form two method is consistent with the traditional classification and molecular classification result, also, clustering analysis using multivariate qualitative morphological characteristics is far more effective than the method using multivariate quantitative characteristics.