Abstract: In order to improve the utilization efficiency of Catalpa bungei genetic resource, fifty three superior clones from gene bank were selected, and more than twelve trait indices were investigated to analyze their genetic diversity. The result showed that there were very significant differences among clones in seedling height and ground diameter, with their genetic variation coefficients were 18.53%and 16.29%, respectively. And genetic diversity indices were 1.84 and 1.79, respectively. Based on the correlative analysis，there were positive correlation among ground diameter, seedling height and leaf length, petiole length, number of leaves, leaf area and dermal pore density, whose genetic variation coefficients were 8.57%, 17.74%, 18.56%, 24.21, 26.91%, respectively. Genetic diversity index were 1.91, 1.87, 1.80, 1.80, 1.94. So they had relatively large variation and were controlled by highly heredity, which had abundant diversity and distributed uniformly.There were big difference among germplasm from different source, among which the growth indexes of germplasm from Henan area were the best. The growth indexes of germplasm from Shandong area were not the best, but its variation coefficients. Fifty three clones were classified four classes by Cluster analysis, in which the ground diameter, height of seedling, number of leaves and dermal pore density of class I were the highest; the performance of class II was the worst. Internode length, leaf length, leaf width, petiole length, leaf area and leaf perimeter of class III were the biggest. Chlorophyll content,specific leaf weight of class IV and class V were the highest, respectively.