Germplasms with high anthocyanin content and antioxidant activity in mulberry are of importance for fruit mulberry breeding. 160 fruit mulberry germplasm resources were screened for evaluation of anthocyanin content and antioxidant activity in mulberry in this study．The results were summarized as follows，the ranges of anthocyanin contents, total antioxidant capacity (TAC), 1，l-Diphenyl-2-picrylhydrazyl radical(DPPH)scavenging ability in 160 fruit mulberry accessions were 106.5-1472.0 mg•L-1, 5.4-32.3 mmol•mL-1 and 33.7-87.8%, respectively, displaying significant varieties differences. The differences between accessions with Ploidies were not significant at 0.05 levels. 160 fruit mulberry accessions could be clustered into 7 clusters which consist of 13, 11, 56, 44, 10 and 26 accessions, respectively. There were significantly positive correlations between the anthocyanin contents and TAC, anthocyanin contents and DPPH. The results showed total anthocyanin in mulberry were the important antioxidation substances. The fruit mulberry accessions with high contents of anthocyanin and antioxidant activity are expected to be used in further quality breeding of fruit mulberry cultivars.